所在院校:北京大学化学院
个人主页:https://biopic.pku.edu.cn/zxcy/zxpi/269931.htm
博士,北京大学生物医学前沿创新中心副主任、研究员。国家杰出青年科学基金获得者,优秀青年科学基金获得者,教育部新世纪优秀人才。
教育工作经历:
2016.8-现在,北大未来基因诊断高精尖创新中心,研究员
2015-现在,北大-清华生命科学联合中心,研究员
2013-现在, 北京大学BIOPIC, 中心副主任
2013-现在,北京大学高通量测序中心常务副主任
2013-现在,北京大学工学院,教授
2010-现在,北京大学BIOPIC,研究员
2007-现在,北京大学化学与分子工程学院,兼职研究员
2006-2013,北京大学工学院,特聘研究员
2005-2006,美国斯坦福大学生物工程系,博士后
2002-2005,美国加州理工学院应用物理系,博士后
1997-2002,北京大学化学学院(分子基功能材料及激光光谱学),理学博士
1993-1997,北京大学化学学院(富勒烯化学),理学学士
主要研究方向:
黄岩谊课题组致力于发展应用于集成生物学研究的新技术。课题组当前的研究兴趣集中在基因测序技术、微流控技术及生物成像技术三个方面。我们一直在发展高效、新型的高通量DNA测序技术及其应用方法,特别是测序化学与设备研发、单细胞测序技术应用与器件研发、以及少量细胞的表观基因组学测序方法研发。我们同时大力发展大规模集成微流控芯片技术,并将其应用在生命科学关键问题的研究中,包括细胞培养及微环境控制,以及在单细胞层次上观察生命过程的随机性。我们还大力发展新的生物成像技术及图像处理算法,用于研究细胞及活生物体的动态过程。我们利用相干拉曼散射显微技术,可以无需标记来研究活生物体和活细胞内的三维化学成像,同时利用这样的技术来促进药物释放及癌症诊断的研究。
获奖及荣誉:
2015,国家自然基金委员会,杰出青年基金
2014, 英国皇家化学会会士
2013,北京大学绿叶生物医学奖
2012,国家自然科学基金委员会,优秀青年基金
2010,第十二届霍英东青年教师基金
2009,北京大学青年教师教学演示竞赛,一等奖
2008,国家教育部新世纪优秀人才
2004,全国优秀博士学位论文
2003,国家自然科学二等奖,第四完成人
2001,北京大学学生五四奖章
2000,中国大学生五四奖学金
代表性论文及论著:
1.Xiannian Zhang, Tianqi Li, Feng Liu, Yaqi Chen, Jiacheng Yao, Zeyao Li, Yanyi Huang, Jianbin Wang. VVV1. Comparative analysis of droplet-based ultra-high-throughput single-cell RNA-seq systems. Molecular Cell. 2019 73(13) 130-142.e5
2.Geng, S (Geng, Shuang); Huang, YY (Huang, Yanyi). From Mouth Pipetting to Microfluidics: The Evolution of Technologies for Picking Healthy Single Cells. ADVANCED BIOSYSTEMS. 2018 2(9)UNSP 1800099.
3.Zhang, Y (Zhang, Yi); Gao, JX (Gao, Jiaxin); Huang, YY (Huang, Yanyi); Wang, JB (Wang, Jianbin). Recent Developments in Single-Cell RNA-Seq of Microorganisms. BIOPHYSICAL JOURNAL, 2018 115(2)173-180.
4.Jiang, Dongqing; Zhang, Xiannian; Pang, Yuhong; Zhang, Jianyun; Wang, Jianbin; Huang, Yanyi. Terminal transfer amplification and sequencing for high-efficiency and low-bias copy number profiling of fragmented DNA samples. Protein & cell. 2018.
5.Chen, H (Chen, He); Yao, JC (Yao, Jiacheng); Fu, YS (Fu, Yusi); Pang, YH (Pang, Yuhong); Wang, JB (Wang, Jianbin); Huang, YY (Huang, Yanyi). Tagmentation on Microbeads: Restore Long-Range DNA Sequence Information Using Next Generation Sequencing with Library Prepared by Surface-Immobilized Transposomes. ACS APPLIED MATERIALS & INTERFACES. 2018 10(14)11539-11545.
6.Chen Z, Liao P, Zhang F, et al. Centrifugal micro-channel array droplet generation for highly parallel digital PCR. Lab Chip 2017;17(2):235-40. doi: 10.1039/c6lc01305h
7.Li S, Chen T, Wang Y, et al. Conjugated Polymer with Intrinsic Alkyne Units for Synergistically Enhanced Raman Imaging in Living Cells. Angew Chem Int Ed Engl 2017;56(43):13455-58. doi: 10.1002/anie.201707042
8.Liao P, Huang Y. Digital PCR: Endless Frontier of ‘Divide and Conquer’. Micromachines 2017;8(8):231.
9.Chen X, Huang Y. Editorial overview: Molecular imaging for seeing chemistry in biology. Curr Opin Chem Biol 2017;39:iv-v. doi: 10.1016/j.cbpa.2017.06.016
10.Chen Z, Zhou W, Qiao S, et al. Highly accurate fluorogenic DNA sequencing with information theory-based error correction. Nat Biotechnol 2017;35(12):1170-78. doi: 10.1038/nbt.3982
11.Chen T, Huang Y. Label-Free Transient Absorption Microscopy for Red Blood Cell Flow Velocity Measurement in Vivo. Anal Chem 2017;89(19):10120-23. doi: 10.1021/acs.analchem.7b01952
12.Zheng C, Zhang X, Li C, et al. Microfluidic Device for Studying Controllable Hydrodynamic Flow Induced Cellular Responses. Anal Chem 2017;89(6):3710-15. doi: 10.1021/acs.analchem.7b00013
13.Yang L, Ma Z, Cao C, et al. MR-seq: measuring a single cell’s transcriptome repeatedly by RNA-seq. Science Bulletin 2017;62(6):391-98. doi: https://doi.org/10.1016/j.scib.2017.01.029
14.Wu AR, Wang J, Streets AM, et al. Single-Cell Transcriptional Analysis. Annu Rev Anal Chem (Palo Alto Calif) 2017;10(1):439-62. doi: 10.1146/annurev-anchem-061516-045228
15.Li C, Yu Z, Fu Y, et al. Single-Cell-Based Platform for Copy Number Variation Profiling through Digital Counting of Amplified Genomic DNA Fragments. ACS Applied Materials & Interfaces 2017;9(16):13958-64. doi: 10.1021/acsami.7b03146
16.Yujiao Dang,# Liying Yan,# Boqiang Hu,# Xiaoying Fan, Yixin Ren, Rong Li, Ying Lian, Jie Yan, Qingqing Li, Yan Zhang, Min Li, Xiulian Ren, Jin Huang, Yuqi Wu, Ping Liu, Lu Wen, Chen Zhang, Yanyi Huang,* Fuchou Tang,* Jie Qiao* .Tracing the expression of circular RNAs in human pre-implantation embryos. Genome Biology 17 130 (2016) .doi: 10.1186/s13059-016-0991-3 (2016) .doi: 10.1021/acs.analchem.6b00862
17.Yusi Fu, Chunmei Li, Sijia Lu, Wenxiong Zhou, Fuchou Tang, X. Sunney Xie,* Yanyi Huang*. Uniform and accurate single-cell sequencing based on emulsion whole-genome amplification. Proceedings of the National Academy of Sciences of the United States of America 112 (38), 11923-11928 (2015) .doi: 10.1073/pnas.1513988112
18.Xiaoying Fan,# Xiannian Zhang,# Xinglong Wu, Hongshan Guo, Yuqiong Hu, Fuchou Tang,* Yanyi Huang*. Single-cell RNA-seq transcriptome analysis of linear and circular RNAs in mouse preimplantation embryos. Genome Biology 16 148 (2015) .doi: 10.1186/s13059-015-0706-1